ONETest™ / Hospital Metagenomics

Advancing Metagenomics for Hospital Labs.

Fusion Genomics develops RUO reagents, software, automation, and FusionCloud™ analytics to support accredited laboratories develop and deploy targeted metagenomic workflows.

Powered by QuantumProbes™, ONETest™ enriches clinically relevant pathogen signal while preserving a representative view of the microbial community.

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ONETest sample-to-result workflow ONETest communication report layout

ONETest™ reagents and software are supplied for research use only and are not intended for diagnostic or therapeutic use. Any clinical use requires independent validation, performance, interpretation, and reporting by an appropriately accredited clinical laboratory under its applicable regulatory, accreditation, and quality framework.

<24h

Sample-to-actionable insight.

50 Families

Family-level core-genome capture spanning bacteria, fungi, and DNA viruses.

300+

Clinically relevant organisms represented in ONETest™ targeted metagenomics.

LDT

RUO reagents & analytics designed to support accredited lab validation.

AI Driven

ML optimized 'Actionable Intelligence'.

The Critical-Care Gap

Hospital teams still face complex infections without timely microbiology.

Culture can be slow and insensitive after antibiotics. PCR is fast but narrow. Untargeted metagenomics is broad, but cost and workflow complexity have limited routine hospital adoption.

Culture PCR Send-out mNGS ONETest
ONETest™ is designed to support broad, efficient targeted metagenomic workflows in accredited laboratory settings.
Integrated Platform

Four technologies support hospital-lab metagenomic workflows.

Fusion controls the enrichment, automation, library preparation, and interpretation layers needed to support accredited laboratories in developing in-house metagenomic workflows.

01

QuantumProbes™

Core-genome capture enriches pathogen signal across covered organisms and related lineages.

02

Automation

Walk-away liquid handling reduces manual complexity and improves reproducibility.

03

UniPrep™

Captured DNA is converted into sequencing-ready libraries on standard infrastructure.

04

FusionCloud™

Actionable Intelligence supports organism prioritization, AMR-marker context, and interpretation evidence.

ONETest integrated workflow
New Class of Targeted Metagenomics

Broad microbial context with enriched target signal.

ONETest™ is not a narrow panel or short-amplicon assay. It uses probe-based core-genome capture to increase pathogen signal while maintaining a representative view of the microbial community.

26×

Normalized on-target microbial abundance versus WmGS.

r=0.74

Shannon diversity concordance with WmGS.

6.2M

QuantumProbes™ in the current panel.

Shannon diversity concordance plot

Microbial community representation

ONETest™ preserves within-sample microbial diversity while enriching targeted pathogen signal.

On-target enrichment plot

Enrichment without losing context

The assay enriches targeted organisms while retaining the broader microbial profile needed for interpretation.

Culture-positive fold-change plot

Higher signal for culture-confirmed species in BAL evaluation

Targeted capture increases per-pathogen signal for many clinically relevant organisms at lower sequencing depth.

Representative plots from the ONETest™ BAL evaluation; full manuscript and preprint materials should be linked through the Resources section.

FusionCloud AI Actionable Intelligence workflow
FusionCloud™ / Fusion AI

Turning genomic signal into structured evidence.

FusionCloud™ applies control-aware filtering, species-specific thresholds, and multi-parameter evidence to qualify, categorize, and rank microbial findings.

  • ✓Background-aware microbial qualification and categorization.
  • ✓Organism-specific dynamic ML cut-offs calibrated for every LDT implementation.
  • ✓Analysis layer for relevance and AMR-marker context.
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Adoption Model

Designed for low-friction hospital deployment.

ONETest™ is built around the practical realities of hospital laboratories: accreditation, IT/Infosec review, workflow integration, physician education, and LDT validation.

01

Clinical seeding

Educate infectious disease, ICU, transplant, and respirology champions around cases where current testing leaves diagnostic gaps.

02

Hub-based testing

Institutions, clinicians, MICU/ICU teams can evaluate ONETest™ workflows through an accredited hub where available and validated.

03

In-house conversion

Once validation, workflow fit, and utilization are established, hospitals can adopt ONETest™ within accredited laboratory workflows.

ONETest™ diagnostic deployment requires an accredited laboratory-developed test framework or other applicable regional/national regulatory pathway.
Resources

Dive deeper into the platform.

Read more about: ONETest™ platform, FusionCloud™, manuscript/preprint, or start a conversation with Fusion.

☷

ONETest™ Platform

See the workflow, QuantumProbes™, UniPrep™, and hospital implementation structure.
AI

FusionCloud™ AI

Explore the interpretation layer for signal qualification and Actionable Intelligence.
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About Fusion

Learn why Fusion Genomics was built to bring sequencing-based diagnostics into hospitals.
✉

Contact Us

Start an implementation discussion, collaboration, investor, or partnership conversation.
Resources & Access

Explore the evidence behind ONETest™.

Access scientific publications, platform resources, and deployment materials for ONETest™ targeted metagenomics and FusionCloud™ / Fusion AI.

01

Evidence & Publications

Peer-reviewed papers, preprints, posters, and clinical validation materials supporting ONETest™ targeted metagenomics.

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Manuscripts & Preprints

Published papers and official preprint links.

PDF

Posters & Presentations

Conference posters and scientific presentations.

02

Platform Resources

Technical briefs and overview materials covering ONETest™, QuantumProbes™, UniPrep™, automation, and FusionCloud™ / Fusion AI.

+

ONETest™ Platform

Targeted metagenomics enabled by QuantumProbes™.

AI

FusionCloud™ / Fusion AI

Interpretation, ranking, and report-ready outputs.

03

Deployment & Access

Implementation resources, validation documentation, and demo access for qualified hospital, laboratory, and strategic partners.

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Hospital Implementation

Qualified lab and hub-based deployment pathways.

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Partner Access

Validation documents and demos by request.

From Dx To Rx in <24 Hours

Closing the critical gap between infection and intervention.

ONETest™ transforms genomic data into actionable intelligence for the hospital laboratory.

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